Article
[Methodological evaluation on PCR-DGGE technique in detecting DNA mutation and single nucleotide polymorphism].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 1 Sept 2007
Lu Xiao-jun, Jia Yong-qian, Fan Hong, Zhang Lei, Jia Jin
Abstract excerpt
OBJECTIVE: To study the efficiency of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) in detecting the DNA base mutation and single nucleotide polymorphism (SNP). METHODS: By DNA sequencing and PCR-DGGE respectively, mutations in the D-loop of the mitochondrial genome were detected in patients with acute leukemia in period of diagnosis and remission, which was regarded as...
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