Three clustered origins of replication in a promiscuous-plasmid replicon and their differential use in a PolA+ strain and a delta PolA strain of Escherichia coli K-12.

doi:10.1128/jb.174.24.8139-8143.1992

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Article

Three clustered origins of replication in a promiscuous-plasmid replicon and their differential use in a PolA+ strain and a delta PolA strain of Escherichia coli K-12.

Journal of bacteriology - 1 Dec 1992

Banerjee S K, Luck B T, Kim H Y, Iyer V N

Abstract excerpt

A 1,197-bp region of the broad-host-range plasmid pCU1 is adequate for its replication. Analysis of replicating molecules containing this region reveals three clustered origins of vegetative replication and replication proceeds bidirectionally from each in a theta mode. In an Escherichia coli pol...

Topics

Cloning, Molecular
DNA Polymerase I
DNA Replication
DNA, Bacterial
Escherichia coli
Microscopy, Electron
Mutation
Plasmids
Replicon

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