Article
Effects of nucleotide substitutions within the T-loop of precursor tRNAs on interaction with ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli and yeast.
The Biochemical journal - 15 Feb 1996
Li Z, Gillis K A, Hegg L A, Zhang J, Thurlow D L
Abstract excerpt
Recognition of tRNA and tRNA-like substrates by the enzyme ATP/CTP:tRNA nucleotidyltransferase requires chemically intact nucleotides within the T-loop, especially at positions 57 and 58, which are invariant purines among naturally occurring tRNAs. To test the effects of base substitutions at the...
Topics
- Adenosine Triphosphate
- Cytidine Triphosphate
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Nucleotidyltransferases
- RNA Precursors
- RNA, Transfer, Glu
- Saccharomyces cerevisiae
- Substrate Specificity