Article
Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.
The Biochemical journal - 15 Feb 1996
Ella K M, Dolan J W, Qi C, Meier K E
Abstract excerpt
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.
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