Article
Isolation and characterization of temperature-sensitive plc1 mutants of the yeast Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 20 Apr 1995
Yoko-o T, Kato H, Matsui Y, Takenawa T, Toh-e A
Abstract excerpt
The PLC1 gene of the yeast Saccharomyces cerevisiae has been discovered to encode a homolog of mammalian phosphoinositide-specific phospholipase C (PLC). Five temperature-sensitive plc1 mutants were isolated by in vitro mutagenesis with subsequent plasmid shuffling. All of the amino acid substitutions that caused a temperature-sensitive growth phenotype were located in the X or the Y region, both of which are...
Topics
- Amino Acid Sequence
- Flow Cytometry
- Genetic Markers
- Isoenzymes
- Molecular Sequence Data
- Mutation
- Phosphatidylinositol Diacylglycerol-Lyase
- Phosphoric Diester Hydrolases
- Plasmids
- Saccharomyces cerevisiae