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Article

Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters.

Journal of bacteriology - 1 Nov 1993

de Lorenzo V, Cases I, Herrero M, Timmis K N

Abstract excerpt

Transcriptional lacZ fusions to the Pu and Pm promoters of the TOL (toluene degradation) plasmid inserted in monocopy in the chromosome of Pseudomonas putida showed a very different responsiveness to their respective aromatic effectors regarding growth phase. While a substantial XylS-dependent activation of Pm-lacZ was detected nearly instantly after m-toluate addition, XylR- and xylene-mediated induction of the...

Topics

Biotransformation
Chromosomes, Bacterial
Cloning, Molecular
DNA Transposable Elements
Escherichia coli
Genes, Bacterial
Mutagenesis, Insertional
Operon
Phenotype
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Pseudomonas putida

Recombinant Fusion Proteins

Restriction Mapping

Toluene

beta-Galactosidase

Source

Open in PubMed Open full text

Identifiers

PMID: 8226632
DOI: 10.1128/jb.175.21.6902-6907.1993
PMCID: PMC206815

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8226632-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters.PMID 8226632Read ↗

VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein.Semantic proximity Read ↗

A T7 RNA polymerase-based system for the construction of Pseudomonas strains with phenotypes dependent on TOL-meta pathway effectors.Semantic proximity Read ↗

Integration of signals through Crc and PtsN in catabolite repression of Pseudomonas putida TOL plasmid pWW0.Semantic proximity Read ↗

Cross talk between catabolic pathways in Pseudomonas putida: XylS-dependent and -independent activation of the TOL meta operon requires the same cis-acting sequences within the Pm promoter.Semantic proximity Read ↗

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Genetic evidence of separate repressor and activator activities of the XylR regulator of the TOL plasmid, pWW0, of Pseudomonas putida.Semantic proximity Read ↗

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Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains.Semantic proximity Read ↗

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A general system to integrate lacZ fusions into the chromosomes of gram-negative eubacteria: regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy.Semantic proximity Read ↗

Metabolism-dependent taxis towards (methyl)phenols is coupled through the most abundant of three polar localized Aer-like proteins of Pseudomonas putida.Semantic proximity Read ↗

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