Article
Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes.
Gene - 15 Jan 1993
de Lorenzo V, Eltis L, Kessler B, Timmis K N
Abstract excerpt
Novel transposon and plasmid-based broad-host-range expression systems have been developed to facilitate the genetic analysis of gene products of Pseudomonas and related Gram- bacteria. The properties of lacIq/Ptrp-lac were used to construct mini-Tn5 expression vector transposons and RSF1010-deri...
Topics
- Bacteria
- Bacterial Proteins
- Cloning, Molecular
- DNA Transposable Elements
- DNA-Binding Proteins
- Dioxygenases
- Genes, Bacterial
- Lac Operon
- Oxygenases
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Pseudomonas
- Restriction Mapping
- Trans-Activators