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Article

Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes.

Gene - 15 Jan 1993

de Lorenzo V, Eltis L, Kessler B, Timmis K N

Abstract excerpt

Novel transposon and plasmid-based broad-host-range expression systems have been developed to facilitate the genetic analysis of gene products of Pseudomonas and related Gram- bacteria. The properties of lacIq/Ptrp-lac were used to construct mini-Tn5 expression vector transposons and RSF1010-derived plasmids for controlled expression and generation of conditional phenotypes. These plasmids were used to...

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PMID: 8380783
DOI: 10.1016/0378-1119(93)90533-9

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