Article
A T7 RNA polymerase-based system for the construction of Pseudomonas strains with phenotypes dependent on TOL-meta pathway effectors.
Gene - 30 Nov 1993
Herrero M, de Lorenzo V, Ensley B, Timmis K N
Abstract excerpt
A general method to construct recombinant Pseudomonas putida (and related bacteria), which transcribe specific genes inserted into their chromosome in response to the presence of alkyl- and halobenzoates, has been developed. The system is based on the ability of the T7 RNA polymerase (T7pol) to i...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Pseudomonas putida
- Trans-Activators
- Viral Proteins