Article
[Physical mapping of Escherichia coli genome rearrangements using pulsed-field gel electrophoresis and the PCR method].
Genetika - 1 Jan 1996
Zaporozhets D A, Mironov A S
Abstract excerpt
Physical mapping of Tn10-induced chromosomal rearrangements in Escherichia coli was carried out by means of pulse-field electrophoresis, Southern hybridization, and PCR analysis. Mutants with chromosomal rearrangements were obtained by a method of selection of strains with constitutive udp-gene expression elaborated earlier. Screenings were performed in two E. coli strains that had a udp gene orientation...
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