Article
[Transposition of the composite synthetic transposon TnV (Tn5-Rep(pSC101)) is accompanied by the formation of the mini-plasmid pTnV, containing defective Is50-elements].
Molekuliarnaia biologiia - 1 Jan 2000
Borovok I A
Abstract excerpt
Using thermoelimination (at 42 degrees C) of the thermoinducible coliphage P1tsCmr omega::TnV (TnV is a Tn5 derivative which contains the replication origin (Rep) of plasmid pSC101), more than 110 KmrCms Escherichia coli K12 clones were selected. It was supposed that the KmrCms phenotype could result only from insertion of TnV (Kmr) into E. coli chromosome and the loss of phage (Cms). It was found that the...
Topics
- Coliphages
- DNA
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Genes, Viral
- Mutation
- Plasmids
- Restriction Mapping