Article
Tn10-mediated inversions fuse uridine phosphorylase (udp) and rRNA genes of Escherichia coli.
Journal of bacteriology - 1 Apr 1994
Fonstein M, Nikolskaya T, Zaporojets D, Nikolsky Y, Kulakauskas S, Mironov A
Abstract excerpt
Two strains carrying metE::Tn10 insertions (upstream of the udp gene) were used to isolate mutants of Escherichia coli overexpressing udp. These strains differ in their gene order; one contains an inversion between the rrnD and rrnE rRNA operons. Selection was based on the ability of overexpressed Udp to complement thymine auxotrophy. Chromosomal rearrangements that connect the udp gene and promoters of different...
Topics
- Base Sequence
- Chromosome Inversion
- DNA Transposable Elements
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Operon