Article
[Thermosensitive vector derived from RP1 plasmid for detection of transposable elements].
Molekuliarnaia biologiia - 1 Jan 2000
Danilevich V N, Amosenko F A, Volozhantseva I Ia, Stepanshin Iu G, Nosikov V V
Abstract excerpt
The involvement of the transposable DNA element of E. coli K12 chromosome in integrative recombination of RP1 plasmid was studied. Using temperature sensitive for replication plasmid RP1ts12--the derivative of RP1 which contains mutated transposon Tnl, it was shown that integration of RP1 into ho...
Topics
- Bacterial Proteins
- Base Composition
- Chromosomes, Bacterial
- DNA Restriction Enzymes
- DNA Transposable Elements
- DNA, Recombinant
- Escherichia coli
- Mutation
- Plasmids
- Rec A Recombinases
- Temperature