Article
[Thermosensitive vector derived from RP1 plasmid for detection of transposable elements].
Molekuliarnaia biologiia - 1 Jan 2000
Danilevich V N, Amosenko F A, Volozhantseva I Ia, Stepanshin Iu G, Nosikov V V
Abstract excerpt
The involvement of the transposable DNA element of E. coli K12 chromosome in integrative recombination of RP1 plasmid was studied. Using temperature sensitive for replication plasmid RP1ts12--the derivative of RP1 which contains mutated transposon Tnl, it was shown that integration of RP1 into host chromosome and Hfr formation may occur according to a mechanism mediated by chromosome IS-elements. Plasmids that...
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