Article
[Integration and intramolecular transposition of the TnBP3 Bordetella pertussis transposon in the Escherichia coli K-12 cells -- mutant for the phosphoenolpyruvate-dependent phosphotransferase system Hpr protein ].
Genetika - 1 Jul 2001
Sivov I G, Bol'shakova T N, Karataev G I
Abstract excerpt
Integration of a plasmid carrying the TnBP3 transposon of Bordetella pertussis into the chromosome of Escherichia coli and transpositions of the integrated structure within a chromosome in the wild-type and mutant cells ptsH devoid of the major Hpr protein of the phosphoenolpyruvate-dependent phosphotransferase system were studied. When transposed to a new chromosome site, the integrated structure was precisely...
Topics
- Bacterial Proteins
- Bordetella pertussis
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Mutation
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Plasmids
- Tandem Repeat Sequences