Article
Real-time analysis of Oct protein-octamer interaction and transcription complex assembly.
Molecular immunology - 1 Dec 1995
Malmborg A C, Johansson K, Sigvardsson M, Borrebaeck C A, Leanderson T
Abstract excerpt
Specific interactions between the protein-binding sequence of the immunoglobulin transcription regulatory element, the octamer, and Oct proteins have been investigated using a biosensor based on surface plasmon resonance. By analysis of in vitro translated Oct1 and Oct2A with a consensus octamer probe, it was shown that the affinity constant, association rate constant and dissociation rate constant of Oct1 were...
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