Article
The dynamic structure of EF-G studied by fusidic acid resistance and internal revertants.
Journal of molecular biology - 10 May 1996
Johanson U, Aevarsson A, Liljas A, Hughes D
Abstract excerpt
We have previously identified 20 different fusidic acid-resistant alleles of fusA, encoding mutant forms of the ribosomal translocase EF-G. One of these, P413L, is used here as the starting point in selections for internal revertants, identifying 20 different pseudo-wild-type forms of EF-G. We ha...
Topics
- Anti-Bacterial Agents
- Binding Sites
- DNA Mutational Analysis
- Directed Molecular Evolution
- Drug Resistance, Microbial
- Escherichia coli
- Fusidic Acid
- Models, Molecular
- Mutation
- Peptide Elongation Factor G
- Peptide Elongation Factors
- Protein Structure, Tertiary
- Protein Synthesis Inhibitors
- RNA, Ribosomal
- Salmonella typhimurium
- Spectinomycin
- Suppression, Genetic
- Thermus thermophilus