Article
A general system to integrate lacZ fusions into the chromosomes of gram-negative eubacteria: regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy.
Molecular & general genetics : MGG - 1 May 1992
Kessler B, de Lorenzo V, Timmis K N
Abstract excerpt
A new procedure is described to recombine plasmid-borne lacZ fusions into the chromosome of gram-negative eubacteria in order to study promoter activity in monocopy. The procedure is based upon the insertion into the chromosome of a target bacterium of a recombinant transposon that carries DNA se...
Topics
- Blotting, Southern
- Chromosomes, Bacterial
- Cloning, Molecular
- Codon
- DNA Transposable Elements
- DNA, Bacterial
- DNA, Recombinant
- Genes, Bacterial
- Genotype
- Gram-Negative Bacteria
- Lac Operon
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Pseudomonas putida