Article
Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse.
Proceedings of the National Academy of Sciences of the United States of America - 2 Apr 1996
Morgan B A, Conlon F L, Manzanares M, Millar J B, Kanuga N, Sharpe J, Krumlauf R, Smith J C, Sedgwick S G
Abstract excerpt
Transposon Tn1000 has been adapted to deliver novel DNA sequences for manipulating recombinant DNA. The transposition procedure for these "tagged" Tn1000s is simple and applicable to most plasmids in current use. For yeast molecular biology, tagged Tn1000s introduce a variety of yeast selective m...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- Cosmids
- DNA Primers
- DNA Transposable Elements
- DNA, Recombinant
- Embryo, Mammalian
- Embryo, Nonmammalian
- Enhancer Elements, Genetic
- Escherichia coli
- Genes, Homeobox
- Genetic Markers
- Genotype
