Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse.

Proceedings of the National Academy of Sciences of the United States of America - 2 Apr 1996

Morgan B A, Conlon F L, Manzanares M, Millar J B, Kanuga N, Sharpe J, Krumlauf R, Smith J C, Sedgwick S G

Abstract excerpt

Transposon Tn1000 has been adapted to deliver novel DNA sequences for manipulating recombinant DNA. The transposition procedure for these "tagged" Tn1000s is simple and applicable to most plasmids in current use. For yeast molecular biology, tagged Tn1000s introduce a variety of yeast selective markers and replication origins into plasmids and cosmids. In addition, the beta-globin minimal promoter and lacZ gene...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Cloning, Molecular

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Primers

Share to DNA Transposable Elements

Share to Escherichia coli

Share to Genetic Markers

Share to Genotype

Source

Open in PubMed Open full text

Identifiers

PMID: 8610121
DOI: 10.1073/pnas.93.7.2801
PMCID: PMC39713

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse.PMID 8610121Read ↗

Creating recombination-activated genes and sequence-defined mutant libraries using transposons.Semantic proximity Read ↗

A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae.Semantic proximity Read ↗

Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions.Semantic proximity Read ↗

Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion.Semantic proximity Read ↗

The Drosophila gene disruption project: progress using transposons with distinctive site specificities.Semantic proximity Read ↗

Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.Semantic proximity Read ↗

In vivo site-directed mutagenesis using oligonucleotides.Semantic proximity Read ↗

Discovery of gene regulatory elements through a new bioinformatics analysis of haploid genetic screensSemantic proximity Read ↗

Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system.Semantic proximity Read ↗

Genetic analysis of Xenopus transcription factor IIIA.Semantic proximity Read ↗

Mutagenesis by reversible promoter insertion to study the activation of NF-kappaB.Semantic proximity Read ↗

BAC modification through serial or simultaneous use of CRE/Lox technology.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.