Km genotyping by polymerase chain reaction (PCR) using allele-specific amplification primers.

doi:10.1016/0379-0738(95)01775-5

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Article

Km genotyping by polymerase chain reaction (PCR) using allele-specific amplification primers.

Forensic science international - 30 Oct 1995

Yamamoto Y, Ishizu H

Abstract excerpt

We developed a method for identifying genetic polymorphisms of the human immunoglobulin kappa light chain, namely the Km allotypes, by allele-specific amplification by means of the polymerase chain reaction (PCR). This procedure consisted of first and second PCR. In the first PCR, the 353 bp frag...

Topics

Alleles
Base Sequence
Blood
DNA Primers
Gene Frequency
Genotype
Hair
Humans
Immunoglobulin Km Allotypes
Immunoglobulin kappa-Chains
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Saliva
Sensitivity and Specificity

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