Article
Genomic organization and sequence of the human NRAMP gene: identification and mapping of a promoter region polymorphism.
Molecular medicine (Cambridge, Mass.) - 1 Jan 1995
Blackwell J M, Barton C H, White J K, Searle S, Baker A M, Williams H, Shaw M A
Abstract excerpt
BACKGROUND: Murine Nramp is a candidate for the macrophage resistance gene Ity/Lsh/Bcg. Sequence analysis of human NRAMP was undertaken to determine its role in man. MATERIALS AND METHODS: A yeast artificial chromosome carrying NRAMP was subcloned and positive clones sequenced. The transcriptional start site was mapped using 5' RACE PCR. Polymorphic variants were amplified by PCR. Linkage analysis was used to map...
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