Article
Intact protein analysis for site-directed mutagenesis overexpression products: plasmid-encoded R67 dihydrofolate reductase.
Analytical biochemistry - 1 Jun 2002
VerBerkmoes Nathan C, Strader Michael B, Smiley R Derike, Howell Elizabeth E, Hurst Gregory B, Hettich Robert L, Stephenson James L
Abstract excerpt
Mass spectrometry is currently the method of choice for the analysis of recombinant protein expression products. By combining proteolytic digestion with peptide mapping and tandem mass spectrometry techniques, verification of site-directed mutagenesis products can be obtained. The proteolytic digestion step converts a purified recombinant protein into a mixture that must be reseparated, thus greatly increasing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.