Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase.

doi:10.1128/jb.174.9.2851-2857.1992

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Article

Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase.

Journal of bacteriology - 1 May 1992

Verwoert I I, Verbree E C, van der Linden K H, Nijkamp H J, Stuitje A R

Abstract excerpt

The Escherichia coli fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase (MCT) was cloned by complementation of a thermosensitive E. coli fabD mutant (fabD89). Expression of the fabD gene in an appropriate E. coli expression vector resulted in an accumulation of the MCT protein of up to 10% of total soluble protein, which was accompanied by an approximately 1,000-fold increase in the MCT...

Topics

3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
Acyl-Carrier Protein S-Malonyltransferase
Acyltransferases
Alcohol Oxidoreductases
Alleles
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Escherichia coli
Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Fatty Acid Synthase, Type II

Fatty Acid Synthases

Genetic Complementation Test

Molecular Sequence Data

Operon

Phosphotransferases

Recombinant Proteins

Sequence Homology, Nucleic Acid

Source

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Identifiers

PMID: 1314802
DOI: 10.1128/jb.174.9.2851-2857.1992
PMCID: PMC205936

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1314802-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase.PMID 1314802Read ↗

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