Article
Rapid mass spectrometric analysis of 15N-Leu incorporation fidelity during preparation of specifically labeled NMR samples.
Protein science : a publication of the Protein Society - 1 Sept 2008
Truhlar Stephanie M E, Cervantes Carla F, Torpey Justin W, Kjaergaard Magnus, Komives Elizabeth A
Abstract excerpt
Advances in NMR spectroscopy have enabled the study of larger proteins that typically have significant overlap in their spectra. Specific (15)N-amino acid incorporation is a powerful tool for reducing spectral overlap and attaining reliable sequential assignments. However, scrambling of the label...
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