Article
Recombination at the coagulase locus in Staphylococcus aureus: plasmid integration and amplification.
Journal of general microbiology - 1 Apr 1993
McDevitt D, Wann E R, Foster T J
Abstract excerpt
The integrating plasmid pCOA18, comprising pUC18 linked to a mutated coagulase (coa) gene from Staphylococcus aureus, and constructed by substituting coa sequences with a tetracycline (Tc)-resistance marker (delta coa::Tcr), was transformed into Staphylococcus aureus RN4220, where it underwent recombination with the chromosomal coa locus. Allele-replacement mutants were recovered at a low frequency directly after...
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