Article
Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus.
FEMS microbiology letters - 1 Jun 1997
Brückner R
Abstract excerpt
A system for high-efficiency gene replacement in Staphylococcus carnosus and Staphylococcus xylosus has been developed, that is based on temperature-sensitive Escherichia coli-Staphylococcus shuttle vectors for fragment delivery and erythromycin resistance cassettes to facilitate selection of genomic copies of disrupted genes. The approach was tested by constructing a phosphotransferase-deficient mutant of S....
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