Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing.

doi:10.1101/gr.4.2.76

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Article

Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing.

PCR methods and applications - 1 Oct 1994

Bautista D, Chaubert P, Bertoncini J, Benhattar J

Abstract excerpt

A simple procedure for direct sequencing of double-stranded PCR products by the dideoxy-termination method has been developed using biotinylated sequencing primers. Sequences of the p53 gene have been obtained from DNA extracted from frozen and formalin-fixed paraffin-embedded cancer tissues. Det...

Topics

Base Sequence
Colorimetry
DNA Primers
Electrophoresis, Agar Gel
Genes, p53
Humans
Luminescent Measurements
Molecular Sequence Data
Mutation
Neoplasms
Polymerase Chain Reaction
Sequence Analysis, DNA

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