Article
Mutation detection by cycle sequencing.
Current protocols in human genetics - 1 May 2001
Thierfelder L
Abstract excerpt
Candidate genes are screened for mutations by a DNA sequencing procedure known as cycle sequencing. First, a segment of the candidate gene is PCR amplified from the genomic DNA of an affected individual. The PCR product is then subjected to multiple rounds of further amplification in a thermal cycler using a heat-stable DNA polymerase in the presence of different dideoxynucleotides and a radiolabeled primer. The...
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