Characterization of yeast plasma membrane H(+)-ATPase mutant pma1-A135V and its revertants.

Article

Characterization of yeast plasma membrane H(+)-ATPase mutant pma1-A135V and its revertants.

The Journal of biological chemistry - 5 Jun 1993

Na S, Perlin D S, Seto-Young D, Wang G, Haber J E

Abstract excerpt

An A135V substitution in the first transmembrane segment of the yeast plasma membrane H(+)-ATPase (PMA1) confers cellular resistance to hygromycin B, exhibits growth sensitivity to low external pH, and results in a defective enzyme that hydrolyzes ATP at 33% of wild type level. The importance of...

Topics

Alleles
Cell Membrane
Cloning, Molecular
Escherichia coli
Glucose
Kinetics
Mutagenesis
Plasmids
Protein Structure, Secondary
Proton-Translocating ATPases
Restriction Mapping
Saccharomyces cerevisiae

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