Article
Modified plant plasma membrane H(+)-ATPase with improved transport coupling efficiency identified by mutant selection in yeast.
The Plant journal : for cell and molecular biology - 1 Sept 1996
Baunsgaard L, Venema K, Axelsen K B, Villalba J M, Welling A, Wollenweber B, Palmgren M G
Abstract excerpt
Transport across the plasma membrane is driven by an electrochemical gradient of H+ ions generated by the plasma membrane proton pump (H(+)-ATPase). Random mutants of Arabidopsis H(+)-ATPase AHA1 were isolated by phenotypic selection of growth of transformed yeast cells in the absence of endogeno...
Topics
- Adenosine Triphosphate
- Arabidopsis
- Biological Transport
- Cell Membrane
- Hydrogen-Ion Concentration
- Hydrolysis
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Proton Pumps
- Proton-Translocating ATPases
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transformation, Genetic
- Tryptophan