Article
Evidence for coupling between membrane and cytoplasmic domains of the yeast plasma membrane H(+)-ATPase. An analysis of intragenic revertants of pma1-105.
The Journal of biological chemistry - 25 Dec 1991
Harris S L, Perlin D S, Seto-Young D, Haber J E
Abstract excerpt
A genetic approach was used to identify interacting portions of the plasma membrane H(+)-ATPase from Saccharomyces cerevisiae. The cellular sensitivity of the pma1-105 strain (S368F) to low external pH and to NH4+ was used to select intragenic revertants of two classes: phenotypically wild-type full revertants and partial revertants that were low pH-resistant but retained resistance to hygromycin B. All 10 full...
Topics
- Adenosine Triphosphate
- Cell Membrane
- Cloning, Molecular
- Cytoplasm
- DNA, Fungal
- Genes, Fungal
- Hydrogen-Ion Concentration
- Hydrolysis
- Mutation
- Phosphorylation
- Protein Conformation