Article
Physiology of mutants with reduced expression of plasma membrane H+-ATPase.
Yeast (Chichester, England) - 1 Jan 2000
Vallejo C G, Serrano R
Abstract excerpt
Two mutations containing insertions and deletions in the promoter in the plasma membrane H+-ATPase gene (PMA1) of Saccharomyces cerevisiae have been introduced into the genome by homologous recombination, replacing the wild-type gene. The resulting strains have 15 and 23% of the wild-type ATPase content. Decreased levels of ATPase correlate with decreased rates of proton efflux and decreased uptake rates of amino...
Topics
- Biological Transport, Active
- Blotting, Southern
- Cell Membrane
- DNA, Fungal
- Gene Expression Regulation
- Glucose
- Hydrogen-Ion Concentration
- Hygromycin B
- Mutation
- Promoter Regions, Genetic
- Proton-Translocating ATPases