Article
Physiology of mutants with reduced expression of plasma membrane H+-ATPase.
Yeast (Chichester, England) - 1 Jan 2000
Vallejo C G, Serrano R
Abstract excerpt
Two mutations containing insertions and deletions in the promoter in the plasma membrane H+-ATPase gene (PMA1) of Saccharomyces cerevisiae have been introduced into the genome by homologous recombination, replacing the wild-type gene. The resulting strains have 15 and 23% of the wild-type ATPase content. Decreased levels of ATPase correlate with decreased rates of proton efflux and decreased uptake rates of amino...
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