Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli.

doi:10.1038/nbt0596-629

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Article

Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli.

Nature biotechnology - 1 May 1996

Simmons L C, Yansura D G

Abstract excerpt

A method for enhancing the secretion of heterologous proteins in Escherichia coli by optimizing, as opposed to simply maximizing, the translational level of a given protein is described. Random alteration of the translational initiation region (TIR) of the Heat-Stable Enterotoxin II (STII) signal...

Topics

Alkaline Phosphatase
Bacterial Proteins
Bacterial Toxins
Base Sequence
Biotechnology
DNA, Bacterial
Enterotoxins
Escherichia coli
Escherichia coli Proteins
Genes, Reporter
Genetic Variation
Genetic Vectors
Molecular Sequence Data
Periplasmic Proteins
Protein Biosynthesis
Recombinant Proteins

Source

Open in PubMed

Identifiers

PMID: 9630956
DOI: 10.1038/nbt0596-629

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9630956-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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