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Article

Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli.

Nature biotechnology - 1 May 1996

Simmons L C, Yansura D G

Abstract excerpt

A method for enhancing the secretion of heterologous proteins in Escherichia coli by optimizing, as opposed to simply maximizing, the translational level of a given protein is described. Random alteration of the translational initiation region (TIR) of the Heat-Stable Enterotoxin II (STII) signal sequence resulted in a library of vectors with varied translational strengths. Subsequent screening of this library...

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PMID: 9630956
DOI: 10.1038/nbt0596-629

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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