Article
Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.
Protein engineering - 1 Jan 2000
Mead D A, Szczesna-Skorupa E, Kemper B
Abstract excerpt
Chimeric phage-plasmid expression vectors were constructed from pUC18/19 plasmids by cloning a single-stranded DNA (ssDNA) origin of replication from bacteriophage f1 and inserting a bacteriophage T7 promoter within the beta-galactosidase gene. A T7 promoter permits in vivo or in vitro expression...
Topics
- Animals
- Base Sequence
- Cattle
- Cloning, Molecular
- DNA, Single-Stranded
- Genetic Vectors
- Helper Viruses
- Molecular Sequence Data
- Mutation
- Parathyroid Hormone
- Plasmids
- Promoter Regions, Genetic
- Protein Engineering
- Protein Precursors
- T-Phages
- Terminator Regions, Genetic