Article
A novel Bacillus subtilis expression vector based on bacteriophage phi 105.
Gene - 2 Nov 1992
Gibson R M, Errington J
Abstract excerpt
We have developed a novel expression vector based on the bacteriophage phi 105, and employed it for the production of mutant beta-lactamases in Bacillus subtilis. Expression of the beta-lactamase-encoding gene was low when cloned into the prophage under the control of its own promoter. However, expression was considerably elevated when the gene was inserted into the phage genome in the same orientation as phage...
Topics
- Bacillus subtilis
- Bacteriophages
- Electrophoresis, Polyacrylamide Gel
- Genes, Bacterial
- Genetic Vectors
- Mutation
- Promoter Regions, Genetic
- beta-Lactamases