Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.

doi:10.1128/jb.175.8.2197-2204.1993

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Article

Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.

Journal of bacteriology - 1 Apr 1993

Khosla C, McDaniel R, Ebert-Khosla S, Torres R, Sherman D H, Bibb M J, Hopwood D A

Abstract excerpt

The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements l...

Topics

Acyl Carrier Protein
Amino Acid Sequence
Anthraquinones
Base Sequence
Molecular Sequence Data
Multienzyme Complexes
Mutation
Recombinant Fusion Proteins

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