Article
Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein.
Molecular microbiology - 1 Nov 1992
Khosla C, Ebert-Khosla S, Hopwood D A
Abstract excerpt
A methodology was developed to construct any desired chromosomal mutation in the gene cluster that encodes the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2). A positive selection marker (resistance gene) is first introduced by double crossing-over into the chromosomal site of interest by use of an unstable delivery plasmid. This marker is subsequently replaced by the desired mutant...
Topics
- Acyl Carrier Protein
- Alleles
- Amino Acid Sequence
- Anthraquinones
- Bacterial Proteins
- Base Sequence
- Fatty Acid Synthases
- Gene Rearrangement
- Genes, Bacterial
- Molecular Sequence Data
- Multienzyme Complexes