Article
Development of a new transformant selection system for Penicillium chrysogenum: isolation and characterization of the P. chrysogenum acetyl-coenzyme A synthetase gene (facA) and its use as a homologous selection marker.
Applied microbiology and biotechnology - 1 Jan 1993
Gouka R J, van Hartingsveldt W, Bovenberg R A, van Zeijl C M, van den Hondel C A, van Gorcom R F
Abstract excerpt
A new transformation system for the filamentous fungus Penicillium chrysogenum is described, based on the use of the homologous acetyl-coenzyme A synthetase (facA) gene as a selection marker. Acetate-non-utilizing (Fac-) strains of P. chrysogenum were obtained by positive selection for spontaneous resistance to fluoroacetate. Among these fac mutants putative facA strains were selected for a loss of...
Topics
- Acetate-CoA Ligase
- Amino Acid Sequence
- Base Sequence
- DNA, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Penicillium chrysogenum
- Transformation, Genetic