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Article

Primary structure of N-linked carbohydrate chains of a human chimeric plasminogen activator K2tu-PA expressed in Chinese hamster ovary cells.

European journal of biochemistry - 15 Mar 1993

Bergwerff A A, van Oostrum J, Asselbergs F A, Bürgi R, Hokke C H, Kamerling J P, Vliegenthart J F

Abstract excerpt

A recombinant human plasminogen activator hybrid variant K2tu-PA, expressed in Chinese hamster ovary cells, is partially glycosylated at Asn12 (A chain, kringle-2 domain) and completely glycosylated at Asn247 (B chain, protease domain). After release of the N-linked carbohydrate chains by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, the oligosaccharides were separated from the protein by gel...

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Identifiers

PMID: 8462541
DOI: 10.1111/j.1432-1033.1993.tb17702.x

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