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Article

Glycosylation of prourokinase produced by Pichia pastoris impairs enzymatic activity but not secretion.

Protein expression and purification - 1 Nov 2000

Wang P, Zhang J, Sun Z, Chen Y, Liu J N

Abstract excerpt

Both glycosylated and nonglycosylated forms of recombinant human prourokinase were produced to the level of 20 mg/L by yeast Pichia pastoris in BMMY medium after 2 days of culture. The expressed pro-UK was 98% secreted into the culture medium and easily purified by carboxymethyl cellulose chromatography. More than 99% of pro-UK in the culture medium was found in single-chain form. This was contradictory to a...

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Identifiers

PMID: 11049742
DOI: 10.1006/prep.2000.1310

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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