Article
Glycosylation of two recombinant human uterine tissue plasminogen activator variants carrying an additional N-glycosylation site in the epidermal-growth-factor-like domain.
European journal of biochemistry - 15 Jan 1994
Pfeiffer G, Strube K H, Schmidt M, Geyer R
Abstract excerpt
Recombinant human uterine tissue plasminogen activator (tPA) glycosylation mutants carrying an additional N-glycosylation site in the epidermal-growth-factor-like domain due to the replacement of either Tyr67 by Asn (YN-tPA) or Gly60 by Ser (GS-tPA) were expressed in mouse epithelial cells (C127)...
Topics
- Amino Acid Sequence
- Asparagine
- Base Sequence
- Carbohydrate Sequence
- Chromatography, High Pressure Liquid
- Female
- Genetic Variation
- Glycine
- Glycopeptides
- Glycosylation
- Humans
- Mass Spectrometry
- Methylation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Oligosaccharides
- Peptide Mapping