Article
Transgenic expression of a variant of human tissue-type plasminogen activator in goat milk: purification and characterization of the recombinant enzyme.
Bio/technology (Nature Publishing Company) - 1 Sept 1991
Denman J, Hayes M, O'Day C, Edmunds T, Bartlett C, Hirani S, Ebert K M, Gordon K, McPherson J M
Abstract excerpt
A glycosylation variant of human tissue-type plasminogen activator (tPA) designated longer-acting tissue-type plasminogen activator (LAtPA) was extensively purified from the milk of a transgenic goat by a combination of acid fractionation, hydrophobic interaction chromatography and immunoaffinity chromatography. This scheme provided greater than 8,000-fold purification of the protein, a cumulative yield of 25%...
Topics
- Animals
- Animals, Genetically Modified
- Carbohydrates
- Electrophoresis, Polyacrylamide Gel
- Female
- Genetic Variation
- Goats
- Humans
- Kinetics
- Milk
- Molecular Weight