The use of sequential high-performance liquid chromatography and capillary zone electrophoresis to separate the glycosylated peptides from recombinant tissue plasminogen activator to a detailed level of microheterogeneity.

Abstract excerpt

The advantage of using the two-dimensional separation method was demonstrated by the separation of the complex tryptic peptides of recombinant human tissue plasminogen activator (rt-PA) glycoprotein. This method hybridizes two analytical methods where fractions containing glycopeptides are collec...

Topics

• Amino Acid Sequence
• Carbohydrate Sequence
• Chromatography, High Pressure Liquid
• Electrophoresis, Capillary
• Genetic Variation
• Glycopeptides
• Glycosylation
• Humans
• Molecular Sequence Data
• Oligosaccharides
• Peptide Fragments
• Peptide Mapping
• Recombinant Proteins
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Tissue Plasminogen Activator
• Trypsin