Carbohydrate structures of a human tissue plasminogen activator variant expressed in recombinant Chinese hamster ovary cells.

doi:10.1016/0014-5793(90)80361-l

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Article

Carbohydrate structures of a human tissue plasminogen activator variant expressed in recombinant Chinese hamster ovary cells.

FEBS letters - 1 Oct 1990

Nimtz M, Noll G, Pâques E P, Conradt H S

Abstract excerpt

The carbohydrate structures of a genetically engineered human tissue plasminogen activator variant bearing a single N-glycosylation site at Asn 448 are reported. After isolation of the tryptic glycopeptide and liberation of the N-linked carbohydrates by polypeptide:N-glycosidase F, 6 major oligosaccharide fractions were separated by HPLC on NH2-bonded phase. Their structures were determined by compositional and...

Topics

Animals
Asparagine
Carbohydrate Conformation
Carbohydrate Sequence
Cell Line
Clone Cells
Cricetinae
Cricetulus
Genetic Variation
Glutamine
Glycoproteins
Glycosylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Methylation

Molecular Sequence Data

Oligosaccharides

Plasminogen Activators

Recombinant Proteins

Source

Open in PubMed

Identifiers

PMID: 2226797
DOI: 10.1016/0014-5793(90)80361-l

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2226797-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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