Article
Gene targeting in the Ig kappa locus: efficient generation of lambda chain-expressing B cells, independent of gene rearrangements in Ig kappa.
The EMBO journal - 1 Mar 1993
Zou Y R, Takeda S, Rajewsky K
Abstract excerpt
The production of lambda chain-expressing B cells was studied in mice in which either the gene encoding the constant region of the kappa chain (C kappa) or the intron enhancer in the Ig kappa locus was inactivated by insertion of a neomycin resistance gene. The two mutants have similar phenotypes...
Topics
- Animals
- B-Lymphocytes
- Base Sequence
- Bone Marrow Cells
- Cell Line
- DNA, Single-Stranded
- Female
- Gene Rearrangement, B-Lymphocyte, Light Chain
- Homozygote
- Immunoglobulin kappa-Chains
- Immunoglobulin lambda-Chains
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutation
- Spleen