Article
Mutant oligonucleotide extension amplification: a nonlabeling polymerase-chain-reaction-based assay for the detection of point mutations.
Acta haematologica - 1 Jan 1991
Efremov D G, Dimovski A J, Janković L, Efremov G D
Abstract excerpt
We have developed a new nonradioactive method for the identification of mutations causing beta-thalassemia and cystic fibrosis. The method is based on the priming of polymerase chain reaction (PCR) by an oligonucleotide complementary to the DNA sequence containing the mutation of interest, which...
Topics
- Base Sequence
- Cystic Fibrosis
- DNA
- Humans
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Polymerase Chain Reaction
- Thalassemia