Article
Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli.
Gene - 30 Jan 1993
Davidson B L, Brown J E, Weber C H, Palella T D, Roessler B J
Abstract excerpt
Naturally occurring mutations in hypoxanthine-guanine phosphoribosyltransferase (HPRT) have been identified by amino acid sequencing, cDNA cloning, and direct nucleotide sequencing of PCR-amplified transcripts. To determine the effect these mutations have on the catalytic properties of the molecu...
Topics
- Base Sequence
- Escherichia coli
- Genetic Vectors
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Recombinant Proteins
- Time Factors