Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The gene for hypoxanthine phosphoribosyl transferase of Plasmodium falciparum complements a bacterial HPT mutation.

Molecular and biochemical parasitology - 1 Jun 1990

Shahabuddin M, Scaife J

Abstract excerpt

The enzyme hypoxanthine phosphoribosyl transferase of Plasmodium falciparum has been overexpressed in Escherichia coli. The protein was found to be active enzymatically. When the recombinant expression vector (pPfPRT2) was transformed and expressed in a Salmonella typhimurium mutant KP1684 (purE deoD hpt gpt), the active expressed protein complemented the hpt mutation in the bacteria. We discuss the practical...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to Escherichia coli
Share to Gene Expression Regulation, Bacterial

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Complementation Test

Share to Hypoxanthine Phosphoribosyltransferase

Share to Mutation

Share to Salmonella typhimurium

Share to Substrate Specificity

Source

Identifiers

PMID: 2204834
DOI: 10.1016/0166-6851(90)90191-n

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The gene for hypoxanthine phosphoribosyl transferase of Plasmodium falciparum complements a bacterial HPT mutation.PMID 2204834Read ↗

Xanthine phosphoribosyltransferase from Leishmania donovani. Molecular cloning, biochemical characterization, and genetic analysis.Semantic proximity Read ↗

Slow ligand-induced conformational switch increases the catalytic rate in Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase.Semantic proximity Read ↗

Biochemical and physiological investigations on adenosine 5’ monophosphate deaminase from Plasmodium spp.Semantic proximity Read ↗

Saturation mutagenesis, complement selection, and steady-state kinetic studies illuminate the roles of invariant residues in active site loop I of the hypoxanthine phosphoribosyltransferase from Trypanosoma cruzi.Semantic proximity Read ↗

Amplification of adenine phosphoribosyltransferase suppresses the conditionally lethal growth and virulence phenotype of Leishmania donovani mutants lacking both hypoxanthine-guanine and xanthine phosphoribosyltransferases.Semantic proximity Read ↗

Identification and characterization of differentiation mutants in the protozoan parasite Toxoplasma gondii.Semantic proximity Read ↗

Expression of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in E. coli.Semantic proximity Read ↗

Acidic residues in the purine binding site govern the 6-oxopurine specificity of the Leishmania donovani xanthine phosphoribosyltransferase.Semantic proximity Read ↗

Product Release Pathways in Human and Plasmodium falciparum Phosphoribosyltransferase.Semantic proximity Read ↗

Molecular characterization and overexpression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma cruziSemantic proximity Read ↗

Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli.Semantic proximity Read ↗

[Cloning, expression and characterization of the hypoxanthine-guanine phosphoribosyltransferase mutants from T. tengcongensis].Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.