Article
Regeneration of three-disulfide mutants of bovine pancreatic ribonuclease A missing the 65-72 disulfide bond: characterization of a minor folding pathway of ribonuclease A and kinetic roles of Cys65 and Cys72.
Biochemistry - 31 Mar 1998
Iwaoka M, Juminaga D, Scheraga H A
Abstract excerpt
The oxidative regeneration pathways of two three-disulfide mutants of bovine pancreatic ribonuclease A (RNase A) missing the 65-72 disulfide bond, [C65S,C72S] and [C65A,C72A], have been studied by using oxidized dithiothreitol (DTTox) as an oxidizing agent and 2-aminoethylmethanethiosulfonate (AEMTS) as a thiol-blocking agent at 25 degrees C and pH 8.0. These mutants are analogues of the des-[65-72] intermediate,...
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