Article
Genetic and biochemical studies of a mutant Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase, nmt72pLeu99-->Pro, that produces temperature-sensitive myristic acid auxotrophy.
The Journal of biological chemistry - 5 Jan 1993
Johnson D R, Duronio R J, Langner C A, Rudnick D A, Gordon J I
Abstract excerpt
Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that transfers myristate from CoA to the amino-terminal glycine residue of at least 12 cellular proteins. Its reaction mechanism is Ordered Bi Bi with myristoyl-CoA binding occurring before bindin...
Topics
- Acyltransferases
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Fungal
- Genotype
- Kinetics
- Leucine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Myristic Acid
- Myristic Acids
- Oligodeoxyribonucleotides