Article
Use of Escherichia coli strains containing fad mutations plus a triple plasmid expression system to study the import of myristate, its activation by Saccharomyces cerevisiae acyl-CoA synthetase, and its utilization by S. cerevisiae myristoyl-CoA:protein N-myristoyltransferase.
The Journal of biological chemistry - 25 Feb 1993
Knoll L J, Gordon J I
Abstract excerpt
A system is described for studying protein N-myristoylation, a eukaryotic protein modification, in Escherichia coli strains containing components of eukaryotic metabolic pathways that regulate metabolism of myristoyl-CoA:protein N-myristoyltransferase (Nmt1p) substrates. Three recombinant plasmid...
Topics
- Acyltransferases
- Bacterial Outer Membrane Proteins
- Biological Transport
- Cloning, Molecular
- Coenzyme A Ligases
- Escherichia coli
- Escherichia coli Proteins
- Fatty Acid Transport Proteins
- Gene Deletion
- Genes, Bacterial