Article
Transposase is the only nematode protein required for in vitro transposition of Tc1.
Genes & development - 15 Mar 1996
Vos J C, De Baere I, Plasterk R H
Abstract excerpt
The Tc1 element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tc1 in a cell-free system. Tc1 appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the Tc1 terminal repeats together with the flanking TA sequence are sufficient for transposition. The target site choice in...
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