Article
Transposase is the only nematode protein required for in vitro transposition of Tc1.
Genes & development - 15 Mar 1996
Vos J C, De Baere I, Plasterk R H
Abstract excerpt
The Tc1 element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tc1 in a cell-free system. Tc1 appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the T...
Topics
- Animals
- Animals, Genetically Modified
- Base Sequence
- Blotting, Southern
- Caenorhabditis elegans
- Cell Nucleus
- Cell-Free System
- DNA Primers
- DNA Transposable Elements
- DNA-Binding Proteins
- Drug Resistance
- Escherichia coli
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Polymerase Chain Reaction
- Recombinant Proteins